10199 Lypd6 Status: Cancelled
| Gene | Lypd6 |
| Chromosome | 2 |
| Deletion Start | 50,165,523 |
| Deletion End | 50,190,817 |
| Deletion Size | 25,295 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 50,166,074 | 50,166,094 | TTCCTACTTCCTGGGCTTTCC |
| TUP | 50,166,096 | 50,166,122 | ATGTCTTTGAACTGTCCAGGGAGCCCA |
| TUR | 50,166,126 | 50,166,144 | ACTGCTGGGACCTGCAATG |
| TDF | 50,190,233 | 50,190,256 | GGTAAGCATTCAGGGTCACAAGAC |
| TDP | 50,190,258 | 50,190,283 | CAAGGCATTGCCATGTCACCCTCACT |
| TDR | 50,190,292 | 50,190,314 | GAGGGTAAAACTTGCCCTTTGAG |
PCR Primers
| Primer | Sequence |
| SU | TATACGGTTCTCCCTTTTGG |
| SD | ATCAGGGTCTGTATCTATTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 328 bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | 327 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
