10201 Fibin Status: Cancelled
| Gene | Fibin |
| Chromosome | 2 |
| Deletion Start | 110,362,792 |
| Deletion End | 110,362,106 |
| Deletion Size | 706 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 110,362,666 | 110,362,687 | TTCGTGCCTGATGGAGACTATG |
| TUP | 110,362,636 | 110,362,658 | TGATGACCCCGAGAAATGCCAGC |
| TUR | 110,362,613 | 110,362,633 | GCGATCACTCACCCTGAAGAG |
| TDF | 110,362,485 | 110,362,503 | TGGAGGGCATCAGCAAGAG |
| TDP | 110,362,425 | 110,362,448 | TGGGACTCCCGCCTCAGGTACTTG |
| TDR | 110,362,404 | 110,362,423 | GAGTAGGCATCCCCGATCTG |
PCR Primers
| Primer | Sequence |
| SU | GTGGAGGAACCAGGATCAG |
| SD | ATGCATCCTTTAGATGGAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 248 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | 323 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
