10202 Lpcat4 Status: ES cell colonies screened / QC no positives
| Gene | Lpcat4 |
| Chromosome | 2 |
| Deletion Start | 112,239,947 |
| Deletion End | 112,246,890 |
| Deletion Size | 6,962 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 112,240,274 | 112,240,293 | TGGGAGAGGGAGCACTGAAG |
| TUP | 112,240,299 | 112,240,326 | CAGTGCCCTCCATCTCTCCTATCCTTGG |
| TUR | 112,240,328 | 112,240,349 | CGTAGGATGTAATCCGCATTCC |
| TDF | 112,246,730 | 112,246,749 | CCCTCCACGACCCTCTGTAC |
| TDP | 112,246,752 | 112,246,777 | CAAGCTTTTCAGCGCCTACCTACGCC |
| TDR | 112,246,783 | 112,246,802 | GAGGTGCTTCGAGGCTTGTG |
PCR Primers
| Primer | Sequence |
| SU | CCCCTCGGCCGGCACCAG |
| SD | CCAGTCCGCTTGTGAATGAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 281 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | 436 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
