10203 Disp2 Status: ES cell colonies screened / QC no positives
| Gene | Disp2 |
| Chromosome | 2 |
| Deletion Start | 118,786,046 |
| Deletion End | 118,792,829 |
| Deletion Size | 6,784 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 118,786,804 | 118,786,823 | CCCCAGCTCACCACCCTTAC |
| TUP | 118,786,825 | 118,786,852 | ACCATACCCAACACCAACTCAGGCATGC |
| TUR | 118,786,878 | 118,786,898 | TGGCCAATCAGCAATCATATG |
| TDF | 118,792,720 | 118,792,738 | GAGGGCCAGGCGATATCAG |
| TDP | 118,792,740 | 118,792,765 | CCTGTGATGCTTCCCAACAGTCAGCC |
| TDR | 118,792,776 | 118,792,795 | GGCCTACGGAGCCAAACATC |
PCR Primers
| Primer | Sequence |
| SU | TCAAGCAGTGCCAGTTATGC |
| SD | GAGTCCTTAGTTGGTCACAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 355 bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 327 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
