| Gene | Tmco5b |
| Chromosome | 2 |
| Deletion Start | 113,286,658 |
| Deletion End | 113,297,009 |
| Deletion Size | 10,352 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,287,695 | 113,287,718 | CCTTGCATCAATTTCTTCATTTCG |
| TUP | 113,287,720 | 113,287,747 | TGACATTTTATTGCACTGTCACTCCGGC |
| TUR | 113,287,750 | 113,287,771 | AAAGGAAGCTGCCACTGAAATC |
| TDF | 113,296,838 | 113,296,859 | CATGATCGTCATCCGGTTACTG |
| TDP | 113,296,865 | 113,296,894 | CGTGTTCTTCCACCTGCAATATGTAAACCC |
| TDR | 113,296,896 | 113,296,916 | CAGGGTGTCCACGAGAAAGTC |
PCR Primers
| Primer | Sequence |
| SU | ATCTGGGAAAGTGAGAGCAC |
| SD | TGAGGATTACATCGAAGTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 269 bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 309 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10204C-G10 | VGB6 | C57BL/6NTac | |
| 10204D-G3 | VGB6 | C57BL/6NTac | |
