10207 Sp9 Status: Cancelled
| Gene | Sp9 |
| Chromosome | 2 |
| Deletion Start | 73,272,055 |
| Deletion End | 73,274,558 |
| Deletion Size | 2,504 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 73,272,785 | 73,272,804 | TTGGGAGCTTCTGGGAGATG |
| TUP | 73,272,807 | 73,272,830 | CGGGCTTATGGCGTCAACCCAAGT |
| TUR | 73,272,837 | 73,272,856 | TCGCTGCCACCCTTGTCTAG |
| TDF | 73,274,295 | 73,274,314 | AACAAGCGTTTCATGCGCAG |
| TDP | 73,274,317 | 73,274,344 | ACCACCTGAGCAAACACATTAAGACGCA |
| TDR | 73,274,376 | 73,274,394 | TGGCGTCCGTGTCACTGTC |
PCR Primers
| Primer | Sequence |
| SU | GGCTCGGGCCCCTCGGAGCG |
| SD | AACCTGCACTTATGTCGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 378 bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | 351 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
