10208 Ttc30a1 Status: ES cell colonies screened / QC no positives
| Gene | Ttc30a1 |
| Chromosome | 2 |
| Deletion Start | 75,981,734 |
| Deletion End | 75,979,720 |
| Deletion Size | 2,015 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,981,696 | 75,981,716 | AAAGTTCCCGACGGTGAGTTC |
| TUP | 75,981,670 | 75,981,694 | CGGATGAGCCTGTACACCACCGCTG |
| TUR | 75,981,641 | 75,981,660 | GCTGCACCGCCTCTGAGTAG |
| TDF | 75,980,613 | 75,980,634 | GACAGCTCCTGAAGAGGCTTTC |
| TDP | 75,980,583 | 75,980,605 | CAGCATGCCAGCCAACCCATCAA |
| TDR | 75,980,555 | 75,980,577 | GCTTAGTGAGTCTCCTGAGCTGC |
PCR Primers
| Primer | Sequence |
| SU | CGGGAGTATAATGTCTGGAC |
| SD | ACGAAAAGATGTGGACGTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 355 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | 332 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
