10213   Trim69     Status: ES cell colonies screened / QC no positives

GeneTrim69
Chromosome2
Deletion Start122,160,875
Deletion End122,178,963
Deletion Size18,089
Genome Build38
MGI IDMGI:1918178
NCBI Gene ID70928
BAC ID462J24
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 2

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF122,161,540122,161,563CCCATCAAGGAATCTCAGTACATG
TUP122,161,565122,161,587TCTGACCCACTCGGCATCCGGAT
TUR122,161,614122,161,635TTCGTGTTCAGCCTCACAGAAG
TDF122,178,777122,178,798CCTCAGAAGAGTGGGCGTGTAC
TDP122,178,800122,178,826AGGATACTTGCCCGCCCTCATAATCCA
TDR122,178,828122,178,850GGGTCATGGTTGTAGCGTTGTAG

PCR Primers

PrimerSequence
SUGATCTCTAGCTTTGGCCTTG
SDTACCCAGAGTACTCTCGTGC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev306 bp-++
TUFTUR96 bp++-
TDFTDR74 bp++-
NeoFwdSD304 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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