10214 Duoxa2 Status: ES cell colonies screened / QC no positives
Gene | Duoxa2 |
Chromosome | 2 |
Deletion Start | 122,299,025 |
Deletion End | 122,302,397 |
Deletion Size | 3,373 |
Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 122,299,100 | 122,299,119 | CAGCGTACCGCTGCTCATTG |
TUP | 122,299,124 | 122,299,152 | AGCTAGCAGCCAAAGACAAGAACACCAGG |
TUR | 122,299,157 | 122,299,177 | CGGATTCCAGGCAAGATGAAC |
TDF | 122,302,130 | 122,302,148 | AGGGATGGGCTCCAAATCC |
TDP | 122,302,154 | 122,302,179 | ATGAGAGCCTGCATCCCTGAGTCCCT |
TDR | 122,302,182 | 122,302,200 | CACCGGAGGGAGGAAAGAC |
PCR Primers
Primer | Sequence |
SU | GCATGACCTCAACCAGACAG |
SD | ACAGTTCTCCTGAGAAATCC |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | 356 bp | - | + | + |
TUF | TUR | 78 bp | + | + | - |
TDF | TDR | 71 bp | + | + | - |
NeoFwd | SD | 256 bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |