10214 Duoxa2 Status: ES cell colonies screened / QC no positives
| Gene | Duoxa2 |
| Chromosome | 2 |
| Deletion Start | 122,299,025 |
| Deletion End | 122,302,397 |
| Deletion Size | 3,373 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 122,299,100 | 122,299,119 | CAGCGTACCGCTGCTCATTG |
| TUP | 122,299,124 | 122,299,152 | AGCTAGCAGCCAAAGACAAGAACACCAGG |
| TUR | 122,299,157 | 122,299,177 | CGGATTCCAGGCAAGATGAAC |
| TDF | 122,302,130 | 122,302,148 | AGGGATGGGCTCCAAATCC |
| TDP | 122,302,154 | 122,302,179 | ATGAGAGCCTGCATCCCTGAGTCCCT |
| TDR | 122,302,182 | 122,302,200 | CACCGGAGGGAGGAAAGAC |
PCR Primers
| Primer | Sequence |
| SU | GCATGACCTCAACCAGACAG |
| SD | ACAGTTCTCCTGAGAAATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 356 bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | 256 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
