10215 Hist1h2ba Status: Cancelled
| Gene | Hist1h2ba |
| Chromosome | 13 |
| Deletion Start | 23,934,153 |
| Deletion End | 23,933,752 |
| Deletion Size | 402 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,934,059 | 23,934,078 | GAGGGCCGGAAACGTAAGAG |
| TUP | 23,934,029 | 23,934,057 | TGCCGCAAGGAGAGCTACTCCATTTACAT |
| TUR | 23,933,982 | 23,934,000 | TGGAGGAGATGCCGGTGTC |
| TDF | 23,933,960 | 23,933,980 | GGCCATGAGCATCATGAACTC |
| TDP | 23,933,928 | 23,933,950 | AGACATCTTCGAGCGCATCGCGA |
| TDR | 23,933,887 | 23,933,907 | GGTCGAGCGCTTGTTGTAATG |
PCR Primers
| Primer | Sequence |
| SU | GATTGGCTGATTGATGATGG |
| SD | GCCCCCAGCTTTATGCGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 336 bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | 310 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
