10216 Tspo2 Status: ES cell colonies screened / QC no positives
| Gene | Tspo2 |
| Chromosome | 17 |
| Deletion Start | 48,450,049 |
| Deletion End | 48,448,604 |
| Deletion Size | 1,446 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 48,449,989 | 48,450,009 | GGCCCATCCTGATTTGTATGC |
| TUP | 48,449,955 | 48,449,979 | TCATCCTCACACCGACTGGATGGCT |
| TUR | 48,449,933 | 48,449,952 | TGGGCACCAGGGTAACTTTC |
| TDF | 48,449,015 | 48,449,034 | GACAGCCCTGGGCTGGTAAG |
| TDP | 48,448,991 | 48,449,012 | ACATGGCAGCCCCATGCTGGGA |
| TDR | 48,448,967 | 48,448,987 | GGGCCCTACGGTTTCACATTC |
PCR Primers
| Primer | Sequence |
| SU | AGTGCTGCTCCGCGGTGTGG |
| SD | CAAGCCATGCATCCACTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 359 bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | 307 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
