10217 Tmem86b Status: Cancelled
| Gene | Tmem86b |
| Chromosome | 7 |
| Deletion Start | 4,630,269 |
| Deletion End | 4,628,352 |
| Deletion Size | 1,918 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,629,732 | 4,629,755 | CCTTTACTTCCTCCTCTGGATTCC |
| TUP | 4,629,703 | 4,629,726 | ACCAGCCATCTTGGGTCAGTGCCC |
| TUR | 4,629,681 | 4,629,701 | AGAATGGGCTGGCACTTGATC |
| TDF | 4,628,857 | 4,628,876 | ACGATGGAGCTGGTGCTTTG |
| TDP | 4,628,826 | 4,628,852 | CTTGGCCACAACCAGACATGAACAGTG |
| TDR | 4,628,805 | 4,628,824 | CCTCCTGCTTCTCCCTGTTG |
PCR Primers
| Primer | Sequence |
| SU | GGGGTGGCCCTTGGTGCCTG |
| SD | AGGGGAAGACAGGATATGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 290 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 310 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
