10219 Chac1 Status: Cancelled
| Gene | Chac1 |
| Chromosome | 2 |
| Deletion Start | 119,351,386 |
| Deletion End | 119,353,590 |
| Deletion Size | 2,205 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 119,351,512 | 119,351,531 | TCGGGTACGGCTCCCTAGTG |
| TUP | 119,351,557 | 119,351,579 | ACGAACGAAGCCCACACGGCTGT |
| TUR | 119,351,581 | 119,351,601 | GCCAGAACCGTCGGCTATAGC |
| TDF | 119,353,460 | 119,353,479 | TGCGTTTGGCAGACTTCATG |
| TDP | 119,353,492 | 119,353,516 | CCTCAGGCACAAGATGAGCACCTGG |
| TDR | 119,353,520 | 119,353,538 | CCTACGGCGTCCACAATGG |
PCR Primers
| Primer | Sequence |
| SU | TGGCCAGAAAGAGATTTGCC |
| SD | CAATGAAGGAAGAGCTGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 333 bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 251 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
