10220 Erlec1 Status: Cancelled
| Gene | Erlec1 |
| Chromosome | 11 |
| Deletion Start | 30,954,072 |
| Deletion End | 30,931,751 |
| Deletion Size | 22,322 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,953,952 | 30,953,970 | CAGCTCAGCGACGACATCC |
| TUP | 30,953,924 | 30,953,948 | TCCGAGTCAACTGGCCTGGTACCGA |
| TUR | 30,953,897 | 30,953,916 | CTCGTGAGGGCACTGACCAG |
PCR Primers
| Primer | Sequence |
| SU | CTCAACAACCCCGGATGTTG |
| SD | TTACCCAAGCAAAGCCAAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 462 bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| NeoFwd | SD | 315 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
