10222 B3galt6 Status: Cancelled
| Gene | B3galt6 |
| Chromosome | 4 |
| Deletion Start | 155,992,613 |
| Deletion End | 155,991,620 |
| Deletion Size | 994 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 155,992,167 | 155,992,188 | GGACTTCGAGTTCGTGCTCAAG |
| TUP | 155,992,111 | 155,992,135 | CTATCCTGGTGGACCTACGCGCACG |
| TUR | 155,992,067 | 155,992,088 | CGGAAAAGAAGCCCCAGTAGAG |
| TDF | 155,991,831 | 155,991,851 | CCACGCTTCGACACGGAGTAC |
| TDP | 155,991,797 | 155,991,824 | TCACCAGATACTGATTGTTGCAGCCTCG |
| TDR | 155,991,775 | 155,991,794 | TCCTCTGGGCTTTGCTTGTG |
PCR Primers
| Primer | Sequence |
| SU | ATATCAGCTCCGGGGAGTCG |
| SD | CAGATGCAATCTCCACATTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 569 bp | - | + | + |
| TUF | TUR | 122 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 301 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
