10223 Ccnb3 Status: Cancelled
| Gene | Ccnb3 |
| Chromosome | X |
| Deletion Start | 7,030,883 |
| Deletion End | 7,005,219 |
| Deletion Size | 25,665 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 7,030,658 | 7,030,681 | TGGAATTTCCAGCAACTCTTGTAG |
| TUP | 7,030,620 | 7,030,647 | TTCTTAGCTCCATGTTCCAACCTGCTGG |
| TUR | 7,030,594 | 7,030,617 | GAGATCATCCCTGAACCAAATTTC |
| TDF | 7,005,549 | 7,005,567 | GGGATGCCTTGTTCCTGGG |
| TDP | 7,005,513 | 7,005,546 | TGCAATCAACATTTGCCTACTATATGCCTGTTCT |
| TDR | 7,005,489 | 7,005,509 | GGCCCTTCAATCTGCAAACTG |
PCR Primers
| Primer | Sequence |
| SU | ATTGTGATAGAATGAAGCTC |
| SD | CCAATAAATGTTATTCACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 303 bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 221 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
