10225 Slc2a10 Status: Cancelled
| Gene | Slc2a10 |
| Chromosome | 2 |
| Deletion Start | 165,504,028 |
| Deletion End | 165,518,242 |
| Deletion Size | 14,215 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 165,504,190 | 165,504,208 | CCCCAGGGCAATGATTCTG |
| TUP | 165,504,214 | 165,504,241 | CATGTCTGTGGAACTATCCCAAGGCCCT |
| TUR | 165,504,245 | 165,504,264 | CAGCCTCAACACCCCAAAGC |
| TDF | 165,517,587 | 165,517,607 | TGACCCACACTCCCAACATTG |
| TDP | 165,517,620 | 165,517,644 | CCATCGGCTTGGCTTGGACCTTCCT |
| TDR | 165,517,646 | 165,517,665 | ACAGCGGTCAGCCCATAGAG |
PCR Primers
| Primer | Sequence |
| SU | GTACCGGGCTACCCTCCGTC |
| SD | ACTGAGGCATGGAGCTAAATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 292 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 300 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
