10226 Cbln3 Status: Germline transmission achieved
| Gene | Cbln3 |
| Chromosome | 14 |
| Deletion Start | 56,503,021 |
| Deletion End | 56,501,798 |
| Deletion Size | 1,224 |
| Genome Build | 37 |
|
|
Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,502,309 | 56,502,329 | AGGTGTACAACCGCCAAACTG |
| TUP | 56,502,279 | 56,502,306 | CAGGTGACCTAAGTGCCAGTCCACCTCC |
| TUR | 56,502,244 | 56,502,264 | TGTGCTGCGGTCCACTATGAC |
| TDF | 56,502,478 | 56,502,497 | CAAATGCCAACCCAGTGATG |
| TDP | 56,502,452 | 56,502,473 | TGAGCTCCCGGCACCCAGAATC |
| TDR | 56,502,403 | 56,502,423 | CTCGCCCTCATTGACCAGTAC |
PCR Primers
| Primer | Sequence |
| SU | ATCCGCAGAGACAGCAGTAG |
| SD | GAGACTGGGAGGGCGACCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 316 bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 295 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10226A-A11 | VGB6 | C57BL/6NTac | |
| 10226A-A7 | VGB6 | C57BL/6NTac | |
| 10226A-E10 | VGB6 | C57BL/6NTac | |
