10230 BC050777 Status: Germline transmission achieved
| Gene | BC050777 |
| Chromosome | 2 |
| Deletion Start | 181,227,995 |
| Deletion End | 181,229,436 |
| Deletion Size | 1,442 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 181,228,215 | 181,228,235 | GGGAGGCGACACACTCACAAG |
| TUP | 181,228,250 | 181,228,274 | CTCGGAGCCAAGTGCAGTGCCTCCT |
| TUR | 181,228,279 | 181,228,297 | TGGGCAGCTCACGGATCTG |
| TDF | 181,228,659 | 181,228,679 | CCCACAACATGATGCAAATGC |
| TDP | 181,228,682 | 181,228,709 | TGCAGCGCATATTTGACCACTACATCCA |
| TDR | 181,228,711 | 181,228,729 | CTGGCGGGAAGTTCAAACG |
PCR Primers
| Primer | Sequence |
| SU | TCCCAGCAACAGTGACTCAC |
| SD | AAGATATACTGCCTACTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 344 bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 53 bp | + | + | - |
| NeoFwd | SD | 219 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10230A-F10 | VGB6 | C57BL/6NTac | |
| 10230B-D2 | VGB6 | C57BL/6NTac | |
| 10230B-A10 | VGB6 | C57BL/6NTac | |
| 10230C-C10 | VGB6 | C57BL/6NTac | |
| 10230C-F6 | VGB6 | C57BL/6NTac | |
| 10230D-H12 | VGB6 | C57BL/6NTac | |
