10232 Slc35e4 Status: Cancelled
| Gene | Slc35e4 |
| Chromosome | 11 |
| Deletion Start | 3,913,184 |
| Deletion End | 3,907,532 |
| Deletion Size | 5,653 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 3,912,987 | 3,913,007 | GCCAGCATGTCAAGCCTCAAC |
| TUP | 3,912,955 | 3,912,979 | TCTTCACAGTGCATGGCTTCGGACG |
| TUR | 3,912,925 | 3,912,945 | AGCATGTGCAGTGCTGAAAGC |
| TDF | 3,907,965 | 3,907,985 | GGTGCTCTGCTGCAAGAAGAG |
| TDP | 3,907,933 | 3,907,957 | ACACGGTGACCCTGCTGTATGCCAC |
| TDR | 3,907,911 | 3,907,930 | CAGGCAGAAACTGGGCAAAG |
PCR Primers
| Primer | Sequence |
| SU | GTGCGGGAGGTGCCCAGGGC |
| SD | ACCCACCATCCAGTGGCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 323 bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | 309 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
