10233 Fam132a Status: Cancelled
| Gene | Fam132a |
| Chromosome | 4 |
| Deletion Start | 155,962,563 |
| Deletion End | 155,966,502 |
| Deletion Size | 3,940 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 155,962,604 | 155,962,623 | CAGCGCTCCTCTGGCTACAG |
| TUP | 155,962,641 | 155,962,665 | CCGAGACTTCTTGAGCTCCTGCCGG |
| TUR | 155,962,669 | 155,962,687 | AATCCACACGCGCAAACAG |
| TDF | 155,966,058 | 155,966,077 | GTTGCCCTGTGGTGCTATGC |
| TDP | 155,966,087 | 155,966,113 | TGCTCTCCAGACCTGATACAGCCTCCA |
| TDR | 155,966,124 | 155,966,144 | CCCTGAACCTGCACTGTGAAG |
PCR Primers
| Primer | Sequence |
| SU | GGAGTTCTGGCTCCTCCTGC |
| SD | CTCTGGGATCACTTATAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 271 bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | 316 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
