10237 Tmem30b Status: Cancelled
| Gene | Tmem30b |
| Chromosome | 12 |
| Deletion Start | 73,546,336 |
| Deletion End | 73,545,259 |
| Deletion Size | 1,078 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 73,546,043 | 73,546,063 | GCTTGCTCCTGGAGCTTTACG |
| TUP | 73,546,005 | 73,546,029 | TCCCGGGCCCCGTGTACCTCTACTA |
| TUR | 73,545,967 | 73,545,985 | CGTAGCGCCGGTTGTTCTG |
| TDF | 73,545,430 | 73,545,449 | CGGCCACAAGCTCATCATTC |
| TDP | 73,545,402 | 73,545,427 | TTGCCACCCATCCAAGAGATGTTGCT |
| TDR | 73,545,378 | 73,545,396 | TAGGCGATGCCCAGGAAAG |
PCR Primers
| Primer | Sequence |
| SU | CAGAGCCTGGTGTGGTGAGG |
| SD | TAAGGACCTGGCGTGTCAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 418 bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 307 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
