10238 1700034O15Rik Status: ES cell colonies screened / QC positives
| Gene | 1700034O15Rik |
| Chromosome | 6 |
| Deletion Start | 41,634,520 |
| Deletion End | 41,635,389 |
| Deletion Size | 870 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 41,635,261 | 41,635,282 | GGTGAGCAGTGGCAGGAGATAG |
| TUP | 41,635,286 | 41,635,312 | TGGTCCAGCAAGAAGACACGATCTCCC |
| TUR | 41,635,315 | 41,635,336 | GGTGGTCCTGGAGAGTTATTGC |
| TDF | 41,634,544 | 41,634,567 | AGAGCAACATTCCTGACAGCTTTG |
| TDP | 41,634,569 | 41,634,595 | CCTTCACTTGGAGCAGCAGCAGTAGCA |
| TDR | 41,634,600 | 41,634,626 | AAGCTTGCACTCACTATCAGAGTCTTC |
PCR Primers
| Primer | Sequence |
| SU | ATGACCATCTCAGATGCCTC |
| SD | TGTCTTTGGCCCCGGGACATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 372 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | 337 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10238B-D2 | VGB6 | C57BL/6NTac | |
| 10238B-D3 | VGB6 | C57BL/6NTac | |
| 10238B-F2 | VGB6 | C57BL/6NTac | |
| 10238B-F9 | VGB6 | C57BL/6NTac | |
| 10238B-G10 | VGB6 | C57BL/6NTac | |
| 10238B-H1 | VGB6 | C57BL/6NTac | |
