10239 Svs6 Status: Cancelled
| Gene | Svs6 |
| Chromosome | 2 |
| Deletion Start | 164,316,751 |
| Deletion End | 164,317,618 |
| Deletion Size | 868 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 164,317,399 | 164,317,418 | TGACAAACACGGCCAAAGTG |
| TUP | 164,317,421 | 164,317,453 | AACTCATGGTGTTCCTTTAAGGCAAATAGTCCA |
| TUR | 164,317,458 | 164,317,480 | ATAAACCTCCTCATGGACAATGC |
| TDF | 164,316,797 | 164,316,818 | AATGCTCCTTGTTCTGGTGACA |
| TDP | 164,316,820 | 164,316,843 | AAACAGCTGCAAAGAGGCCCCGTG |
| TDR | 164,332,857 | 164,332,879 | CTACAGAACGAAATTGCCACTCA |
PCR Primers
| Primer | Sequence |
| SU | AAAGTAAAAGGGAAGATTCC |
| SD | AGACATGGTCACACCTCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 18564 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 16083 bp | + | + | - |
| NeoFwd | SD | 308 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
