10240 Samd10 Status: ES cell colonies screened / QC no positives
| Gene | Samd10 |
| Chromosome | 2 |
| Deletion Start | 181,599,019 |
| Deletion End | 181,596,564 |
| Deletion Size | 2,456 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 181,598,759 | 181,598,778 | GCCTTTGTCCAGCGTTATGG |
| TUP | 181,598,729 | 181,598,752 | AGGCCTGAGAATAACCGCCGCCCT |
| TUR | 181,598,707 | 181,598,727 | CAGTCATCCCCACTCCAAGTC |
| TDF | 181,596,897 | 181,596,917 | GCCTCACTGCCTTCCCTTCTC |
| TDP | 181,596,869 | 181,596,893 | CCCTAGGCCGAGCTCTGCTTCGACT |
| TDR | 181,596,841 | 181,596,863 | CCCCATTCTCTGCAGCTTATCTG |
PCR Primers
| Primer | Sequence |
| SU | GGCTTGGGCTTGGCCGGCTG |
| SD | GCTTGGATCTCAGAAGAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 233 bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 329 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
