10242   Ly6k     Status: ES cell colonies screened / QC no positives

GeneLy6k
Chromosome15
Deletion Start74,799,473
Deletion End74,796,945
Deletion Size2,529
Genome Build38
MGI IDMGI:1923736
NCBI Gene ID76486
BAC ID432F9
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 15

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF74,798,93474,798,955GGGTTGAAAGTAAAGGGTGGTG
TUP74,798,90674,798,933TGAGAATGGATGTCCTCTGGCATCTTGA
TUR74,798,85574,798,878CACATCTGGAAACACTGCATGAAC
TDF74,797,57874,797,597TGCAATGCAAGGTGAGGTTC
TDP74,797,55274,797,576AAGTCTAGCCTCCCACCTGCAGCTA
TDR74,797,52174,797,539CCTACGGTGAGGGCTTCTG

PCR Primers

PrimerSequence
SUGACTATCTGGGGTAGAAAGTG
SDGGAGTTGCTGATGCTGACAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev338 bp-++
TUFTUR101 bp++-
TDFTDR77 bp++-
NeoFwdSD251 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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