10243 1700003F12Rik Status: ES cell colonies screened / QC positives
| Gene | 1700003F12Rik |
| Chromosome | 2 |
| Deletion Start | 154,374,662 |
| Deletion End | 154,375,700 |
| Deletion Size | 1,039 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 154,374,754 | 154,374,773 | AAGCCCGTCACAAGCAGTTC |
| TUP | 154,374,775 | 154,374,800 | TCAGCCATCTCAAGAGGAAGAAGCCA |
| TUR | 154,374,808 | 154,374,829 | GTCCCTTCTAGCCTCTCCATAC |
| TDF | 154,375,271 | 154,375,290 | CCCAGACCAAGATCGTGCTG |
| TDP | 154,375,291 | 154,375,314 | CTTTTCCCGCTGGACAAGCGGCAG |
| TDR | 154,375,340 | 154,375,357 | GCACCGGCCTCACAAATG |
PCR Primers
| Primer | Sequence |
| SU | CTAGCCCTGTTTTGCAGCTG |
| SD | TCAAGTCCACCTTCAGTCTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 356 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | 291 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10243A-C10 | VGB6 | C57BL/6NTac | |
| 10243A-E8 | VGB6 | C57BL/6NTac | |
