10244 Psca Status: Cancelled
| Gene | Psca |
| Chromosome | 15 |
| Deletion Start | 74,714,840 |
| Deletion End | 74,716,691 |
| Deletion Size | 1,852 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 74,714,884 | 74,714,904 | CACCTACTTAGCCCTGCATCC |
| TUP | 74,714,908 | 74,714,932 | TAAGCTTCAGACAGCCACGGGACAG |
| TUR | 74,714,937 | 74,714,961 | GACATTTCTCCATCACTCCTTTCTG |
| TDF | 74,716,218 | 74,716,239 | CCACTCCAGCCAGACATCAAAG |
| TDP | 74,716,242 | 74,716,269 | TTGCTCACCCATTCACCAACCATTTCTG |
| TDR | 74,716,270 | 74,716,289 | TGGACCCACTCCACAAAGAC |
PCR Primers
| Primer | Sequence |
| SU | GCTTCTAGCAAAGACAGCTTG |
| SD | CCTTCAGCTGAAGCATCTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 358 bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 233 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
