10245 Oxct2a Status: ES cell colonies screened / QC no positives
| Gene | Oxct2a |
| Chromosome | 4 |
| Deletion Start | 123,323,583 |
| Deletion End | 123,321,983 |
| Deletion Size | 1,601 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 123,323,338 | 123,323,356 | GAAGACCAAGGGCGTGAAG |
| TUP | 123,323,310 | 123,323,335 | CCTGAAGATCGTCAGCAGCAACGTGG |
| TUR | 123,323,282 | 123,323,301 | AAGATGCCCAGGCCGAAGTC |
| TDF | 123,322,372 | 123,322,393 | GGCCATGCAGGTTTCTCAATAC |
| TDP | 123,322,345 | 123,322,365 | CTGGCCAACTGGATGGTGCCA |
| TDR | 123,322,322 | 123,322,342 | CCCATGCCCTTCACCTTCTTG |
PCR Primers
| Primer | Sequence |
| SU | GGTACAATGGAGTCCCAAAC |
| SD | CTCCATGTTTCCTGACTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 352 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 352 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
