10246 Tmem158 Status: ES cell colonies screened / QC no positives
| Gene | Tmem158 |
| Chromosome | 9 |
| Deletion Start | 123,260,542 |
| Deletion End | 123,259,645 |
| Deletion Size | 898 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 123,260,309 | 123,260,329 | AACATCAGCGTGCAACGACAG |
| TUP | 123,260,287 | 123,260,308 | ATGCTGAGCTCGCTGCTCGTGC |
| TUR | 123,260,229 | 123,260,248 | TGCGCGTTGGTAGAGAAGAG |
| TDF | 123,259,926 | 123,259,945 | CGCAAGCCCATCGAGTCTAC |
| TDP | 123,259,902 | 123,259,923 | TGGTGGCCTGCTTCATGACCCT |
| TDR | 123,259,881 | 123,259,900 | ACGCTCCACACCACGATGAC |
PCR Primers
| Primer | Sequence |
| SU | AGCTCTGGGGGCAGCGGAAC |
| SD | CTTCAGCCCGAGAGCTTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 308 bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | 262 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
