10247   Mageb3     Status: ES cell colonies screened / QC no positives

GeneMageb3
Chromosome2
Deletion Start121,955,216
Deletion End121,954,209
Deletion Size1,008
Genome Build38
MGI IDMGI:105109
NCBI Gene ID17147
BAC ID307A14
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 2

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF121,955,147121,955,167CACGCAGGGAGGTTCAAGTAG
TUP121,955,123121,955,145TCAGCCCACTGCAGAGGAAGCAG
TUR121,955,094121,955,113CCCAGCACTCAGGTCAACAG
TDF121,954,332121,954,353CCCTAGTGCCTTCCCTAATCTC
TDP121,954,305121,954,330ACCAGTTGGCTCTTAGAGATCAGGCA
TDR121,954,268121,954,288CACCCTTGCCTTGAACTCTAC

PCR Primers

PrimerSequence
SUGTCTAGATAGCACTTTTGAC
SDGACAGTTACTTCTGTATGCAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev313 bp-++
TUFTUR74 bp++-
TDFTDR86 bp++-
NeoFwdSD287 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
Larger Image

VelociGene Genotyping Strategy

Larger Image