| Gene | Hist1h2ak |
| Chromosome | 13 |
| Deletion Start | |
| Deletion End | |
| Deletion Size | |
| Genome Build | 37 |
|
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,753,649 | 21,753,668 | GCCGTGCTAGAGTACCTGAC |
| TUP | 21,716,606 | 21,716,628 | AGATCCTGGAGCTGGCGGGCAAC |
| TUR | 21,753,591 | 21,753,611 | AATGCGCGTCTTCTTGTTGTC |
| TDF | 21,716,765 | 21,716,783 | TGCTGCTGCCCAAGAAGAC |
| TDP | 21,753,443 | 21,753,465 | CGAGACCCACCACAAGGCCAAGG |
| TDR | 21,753,418 | 21,753,438 | GCTTTCTATCAAGGGCCTCAC |
PCR Primers
| Primer | Sequence |
| SU | TTGTGGCTATAGCTATCGCG |
| SD | CCTAGAATGCACTACAGGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 36674 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
