10251   4933402P03Rik     Status: Cancelled

Gene4933402P03Rik
Chromosome11
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:1918293
NCBI Gene ID108803
BAC ID402M7
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF69,817,68469,817,701TCAGCCACCAGCAGAGAC
TUP69,817,66169,817,681CGCAACAGGCCACTCTCCCGT
TUR69,817,60369,817,625TTGGCCTCATCTGTGACATCTTG
TDF69,816,78869,816,808CCAACCACCTTTGTTCCACTC
TDP69,816,76369,816,787AGTCGGAATCCAGGTGGCAATGCCA
TDR69,816,73369,816,752TCAGCTCCAGGCTGTCATAC

PCR Primers

PrimerSequence
SUGGTCTCATGACTGCAGCAAC
SDTCTTGAGCAGAGCCGAGTC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR99 bp++-
TDFTDR76 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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