| Gene | Mup2 |
| Chromosome | 4 |
| Deletion Start | |
| Deletion End | |
| Deletion Size | |
| Genome Build | 37 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 62,053,724 | 62,053,747 | AAGTATTGTGACAACTGGCATGAG |
| TUP | 61,857,539 | 61,857,562 | ACCCTTGTGTATGCTCAGGCCCTA |
| TUR | 61,857,512 | 61,857,535 | TGTCCACTTGAACTCCATCCTATG |
| TDF | 62,029,640 | 62,029,662 | TGCTACACTCACTATTGCTCTTC |
| TDP | 61,779,658 | 61,779,683 | TCACAGGCCGAGAACCAGATTTGAGT |
| TDR | 61,779,633 | 61,779,654 | GTGCAAACCTTTCCTTGATGTC |
PCR Primers
| Primer | Sequence |
| SU | CTGACAGAGGTAGAGGAGAC |
| SD | AGCCAATCTTCAAGCTTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 196236 bp | + | + | - |
| TDF | TDR | 250030 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
