10258 Trp53inp2 Status: ES cell colonies screened / QC no positives
| Gene | Trp53inp2 |
| Chromosome | 2 |
| Deletion Start | 155,385,326 |
| Deletion End | 155,386,798 |
| Deletion Size | 1,473 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 155,385,441 | 155,385,461 | GCTGGCTCATCATCGACCTAC |
| TUP | 155,385,466 | 155,385,489 | TGAGGCTTGAGTCAGGATCCAGCA |
| TUR | 155,385,519 | 155,385,537 | TGCAACACCTCCTGTCCTC |
| TDF | 155,386,085 | 155,386,102 | TCACCGGCAGCACCATAG |
| TDP | 155,386,103 | 155,386,124 | TGCTGGAGTCTGGGCCACCTTC |
| TDR | 155,386,133 | 155,386,151 | TCAGGCAAGGCAGCTTCAG |
PCR Primers
| Primer | Sequence |
| SU | CCCAGCACCTTTCTAAGTGC |
| SD | AAGCATGGGAGAGTGGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 345 bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | 357 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
