10259 Hsd3b1 Status: Cancelled
| Gene | Hsd3b1 |
| Chromosome | 3 |
| Deletion Start | 98,857,961 |
| Deletion End | 98,852,040 |
| Deletion Size | 5,941 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | CAGGAGCAGGAGGGTTTGTG |
| TUP | | | CAGAGGATCATCAAGATGTTGGTGCAGGA |
| TUR | | | CCTTGGTTTCTGGTCGGAATAC |
| TDF | | | TCTGGGCTATGAGCCACTTG |
| TDP | | | TCAACTGGGAGGAAGCAAAGCAGAAA |
| TDR | | | TGCTGTTCCACTATTGTCCCTATC |
PCR Primers
| Primer | Sequence |
| SU | TCCAGACACTCTCATCTGAC |
| SD | ACACAGGACATAAAACTTGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 340 bp | - | + | + |
| NeoFwd | SD | 422 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
