10260 1110067D22Rik Status: Germline transmission achieved
| Gene | 1110067D22Rik |
| Chromosome | 11 |
| Deletion Start | 20,731,009 |
| Deletion End | 20,726,329 |
| Deletion Size | 4,681 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,730,308 | 20,730,330 | AAACAACTCCTTGGGTTCTCCAG |
| TUP | 20,730,283 | 20,730,306 | TCAAGCCGACGTGTACTTCCCACG |
| TUR | 20,730,252 | 20,730,272 | GCAGAGACACGGAGCACATTG |
| TDF | 20,726,873 | 20,726,895 | TGTTTGGCATCAAGTGTCATGTG |
| TDP | 20,726,840 | 20,726,866 | TTGGTGCTCCTTCACACCTCTACCTGG |
| TDR | 20,726,789 | 20,726,811 | TCACCACTCCATACCAGTGAAAG |
PCR Primers
| Primer | Sequence |
| SU | CAGGGCACGCTTAGCAAGC |
| SD | CTGGGTATAATGGCTCAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 404 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | 275 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10260A-E2 | VGB6 | C57BL/6NTac | |
| 10260B-C1 | VGB6 | C57BL/6NTac | |
| 10260B-E9 | VGB6 | C57BL/6NTac | |
