10261 Myh8 Status: Cancelled
| Gene | Myh8 |
| Chromosome | 11 |
| Deletion Start | 67,278,860 |
| Deletion End | 67,308,483 |
| Deletion Size | 29,644 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 67,278,984 | 67,279,004 | CGGAGCCCAAGGAGTCTTATG |
| TUP | 67,279,018 | 67,279,045 | ACAAAGCAAGGATGGAGGGAAAGTGACC |
| TUR | 67,279,067 | 67,279,088 | GGCCCTCTGTGTTTTCACTTAC |
| TDF | 67,308,290 | 67,308,308 | GAGGCCCGCTACATCTAAC |
| TDP | 67,308,309 | 67,308,334 | TGCCAGAACGCTTGTCTGTTCTCAGG |
| TDR | 67,308,340 | 67,308,359 | GCCAGGTTGGCATTGGATTG |
PCR Primers
| Primer | Sequence |
| SU | AAGCTGTAGGTACCTACTCC |
| SD | GTGAGAACAAGCTTTGCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 350 bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | 286 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
