10270 Rprml Status: ES cell colonies screened / QC no positives
| Gene | Rprml |
| Chromosome | 11 |
| Deletion Start | 103,649,766 |
| Deletion End | 103,650,152 |
| Deletion Size | 387 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 103,649,835 | 103,649,852 | CGAGCCACCCTGGGAAAC |
| TUP | 103,649,855 | 103,649,872 | CAGCCACGGGCTGGGCAC |
| TUR | 103,649,943 | 103,649,960 | CCACAGGCTGCGTTCGTC |
| TDF | 103,650,000 | 103,650,017 | TCGCTCACCGTGGTCTTC |
| TDP | 103,650,023 | 103,650,048 | TTTCTTCCTGGGCTGTAACCTGCTCA |
| TDR | 103,650,071 | 103,650,089 | GCGCTCCTGCATCAGAAAG |
PCR Primers
| Primer | Sequence |
| SU | CAGTCTCGGAGCTCCGTGG |
| SD | ACTCTGCCCCGAACTTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 390 bp | - | + | + |
| TUF | TUR | 126 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | 315 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
