10271 Lime1 Status: Cancelled
| Gene | Lime1 |
| Chromosome | 2 |
| Deletion Start | 181,382,201 |
| Deletion End | 181,383,462 |
| Deletion Size | 1,262 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 181,382,271 | 181,382,290 | TCCCTGCTCCTTTGGCTATG |
| TUP | 181,382,298 | 181,382,321 | TGCACAGCCTGCCACAGGTATGTG |
| TUR | 181,382,327 | 181,382,347 | AGGGTGAGGGCAGACAGAATC |
| TDF | 181,383,088 | 181,383,109 | CCAGAAGCACAAAGGGACAGAG |
| TDP | 181,383,116 | 181,383,140 | TGCCAAGAATTGCAGCAGAAGGCTA |
| TDR | 181,383,161 | 181,383,180 | CCTACCCAGGCAGACGTTAC |
PCR Primers
| Primer | Sequence |
| SU | CTCTATCTCCATCTCCATTCC |
| SD | GACATCTCTAATCCTTTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 384 bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | 343 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
