10273 2310002L09Rik Status: ES cell colonies screened / QC no positives
| Gene | 2310002L09Rik |
| Chromosome | 4 |
| Deletion Start | 73,950,696 |
| Deletion End | 73,939,561 |
| Deletion Size | 11,136 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 73,950,671 | 73,950,690 | TGCTTGGTGAAGATCAGGAC |
| TUP | 73,950,645 | 73,950,670 | TCAACGAAGCCTCCATTTGCACATGA |
| TUR | 73,950,604 | 73,950,624 | TTTCAGCAGACGGACAAACAC |
| TDF | 73,939,778 | 73,939,801 | CCCACAACATTGCCAGTAAAGAAC |
| TDP | 73,939,745 | 73,939,773 | ACTCAGTCATGCTTTCACCTCTGGAGTGA |
| TDR | 73,939,699 | 73,939,720 | TTACCCAAAGATGCTCAAGTCC |
PCR Primers
| Primer | Sequence |
| SU | CCTCGTTGCCCAATGTCTG |
| SD | CATAAGGCCACCTTTTCAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 337 bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | 340 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
