10276   Txndc17     Status: ES cell colonies screened / QC no positives

GeneTxndc17
Chromosome11
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:1289248
NCBI Gene ID52700
BAC ID438I12
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUFCCACAGCAGTGCTAGGAAGAAG
TUPCCCTGCTGAAGTCAGAAAGTGGGATGA
TURGCAGGACCAAGATCCTCATC
TDFTGACCTTGCCGGACTGTG
TDPTGGCACATGCCTTTAGTCCCAGCA
TDRGACCAGGTAGCCTCAAACTCAAAG

PCR Primers

PrimerSequence
SUGTCACTGTCCATGAAGCCAG
SDCTAATTGCTGCAGATTTGGAAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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