10282 Miip Status: Cancelled
| Gene | Miip |
| Chromosome | 4 |
| Deletion Start | 147,866,345 |
| Deletion End | 147,861,014 |
| Deletion Size | 5,351 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 147,865,859 | 147,865,881 | CGTTTGATGTGGCCTGGTATGTC |
| TUP | 147,865,827 | 147,865,854 | TCAACTCCAGGATGGACTCTTTCCTACC |
| TUR | 147,865,806 | 147,865,823 | TCCTGGTGTTGGCCTGTG |
| TDF | 147,863,798 | 147,863,819 | CATGGTCTCAGCCTGACTGTTC |
| TDP | 147,863,774 | 147,863,796 | TCACATGCCAAGCTCCCGACTGT |
| TDR | 147,863,745 | 147,863,767 | CCCAGTAGGTGAAGGTGTGAAAG |
PCR Primers
| Primer | Sequence |
| SU | TTAGATTACATAGGGAAGGC |
| SD | GGAAGTGTAGGACCCTGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 354 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | 329 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
