| Gene | Rsad1 |
| Chromosome | 11 |
| Deletion Start | 94,549,114 |
| Deletion End | 94,542,511 |
| Deletion Size | 6,604 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 94,548,927 | 94,548,947 | CCGTGGCTGTTCCGTTCATAG |
| TUP | 94,548,892 | 94,548,918 | CACGCTGTCCGTGCCTAACATTACATC |
| TUR | 94,548,869 | 94,548,886 | TGACGCGGGCAGATGTTG |
| TDF | 94,542,969 | 94,542,986 | AGCACTGGCAGCAGTTTG |
| TDP | 94,542,944 | 94,542,964 | CCAGCTGACCCTGTGGGACGT |
| TDR | 94,542,910 | 94,542,927 | TAGCAGCTCCTCCACCTC |
PCR Primers
| Primer | Sequence |
| SU | GTGGGCTCTCTAGCCACTC |
| SD | GTGAGGACATCCTAGGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 270 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 321 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10291A-H7 | VGB6 | C57BL/6NTac | |
| 10291F-E5 | VGB6 | C57BL/6NTac | |
