10292 Fgfbp1 Status: Cancelled
| Gene | Fgfbp1 |
| Chromosome | 5 |
| Deletion Start | 43,979,945 |
| Deletion End | 43,979,174 |
| Deletion Size | 772 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,979,810 | 43,979,829 | GGGTTCAGCTCCCTCGTTAG |
| TUP | 43,979,780 | 43,979,807 | AAGGCCCAGAATAAGCAGAGAAGCAGGA |
| TUR | 43,979,725 | 43,979,745 | CTGCATGTGGCTTGGTCTTTG |
| TDF | 43,979,406 | 43,979,424 | CGCAAGGGAGCACAACAAG |
| TDP | 43,979,381 | 43,979,404 | TCCAAGAAGCTGTCTCCACGGAGC |
| TDR | 43,979,344 | 43,979,366 | GCTGGATTGAGTGTGATGTCTTC |
PCR Primers
| Primer | Sequence |
| SU | ATGGGGCAATAACCTTCAGG |
| SD | ATTGATCCACTACTTATATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 411 bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | 340 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
