10293 Cxcl5 Status: Cancelled
| Gene | Cxcl5 |
| Chromosome | 5 |
| Deletion Start | 90,759,424 |
| Deletion End | 90,760,513 |
| Deletion Size | 1,090 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 90,759,794 | 90,759,812 | CAGGTCCACAGTGCCCTAC |
| TUP | 90,759,822 | 90,759,850 | CATGTGAGTTCTGACTGTGTTCTGGGTGC |
| TUR | 90,759,853 | 90,759,875 | GGACTTCCATCTCCCAAGATCAC |
| TDF | 90,760,044 | 90,760,066 | TGGGCAGGTACTTGCTATCTTAG |
| TDP | 90,760,069 | 90,760,091 | TCTGCAGCGGGCAAGGTCCTTAT |
| TDR | 90,760,097 | 90,760,119 | GATGGGAGCCTCAACTCATACAG |
PCR Primers
| Primer | Sequence |
| SU | CACTCCTCCTAGCCATCCTC |
| SD | ATTTTGAAAGGTTAACAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 364 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 331 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
