| Gene | BC100451 |
| Chromosome | 11 |
| Deletion Start | 118,334,013 |
| Deletion End | 118,332,391 |
| Deletion Size | 1,623 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 118,333,910 | 118,333,928 | CAGCCCAAGAGGAGGACAG |
| TUP | 118,333,882 | 118,333,906 | CACTCTGCAGCAGTGGAAAGCACGG |
| TUR | 118,333,839 | 118,333,860 | CCTGCCACTCTGCCTTTAACTC |
| TDF | 118,332,829 | 118,332,848 | CGCCCAAGTCACCTTTGTTG |
| TDP | 118,332,800 | 118,332,824 | ACGAAAGCCTCAAGCCTTACCTGCA |
| TDR | 118,332,779 | 118,332,799 | GCTACAGAGTCTGCCTGTTTC |
PCR Primers
| Primer | Sequence |
| SU | AGTGGGCAGAGGTTGGGGAG |
| SD | CTGGGGTTTCCATGAGTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 341 bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | 281 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10296A-G2 | VGB6 | C57BL/6NTac | |
| 10296B-H3 | VGB6 | C57BL/6NTac | |
| 10296B-B12 | VGB6 | C57BL/6NTac | |
