Gene | Pramef12 |
Chromosome | 4 |
Deletion Start | 144,395,969 |
Deletion End | 144,392,570 |
Deletion Size | 3,400 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 144,395,123 | 144,395,143 | GTTCTGGATTTGCGGGCTTTG |
TUP | 144,395,097 | 144,395,122 | CACAAGGACTTCTGGACCGTATGGGC |
TUR | 144,395,071 | 144,395,092 | GAGCGCAAGATGTTGTCGTATC |
TDF | 144,393,012 | 144,393,031 | GCATCCGACAGCTCAAACAC |
TDP | 144,392,984 | 144,393,010 | TGAACCTGAGTGGTGTCATTCTGACCG |
TDR | 144,392,957 | 144,392,975 | GGACTCGAAGAGGCTCAGG |
PCR Primers
Primer | Sequence |
SU | ACTTCCTGCTTCCTTGGAGC |
SD | CCACCCATGAAGTAAACACC |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | 354 bp | - | + | + |
TUF | TUR | 73 bp | + | + | - |
TDF | TDR | 75 bp | + | + | - |
NeoFwd | SD | 369 bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
10301E-A6 | VGB6 | C57BL/6NTac | |
10301E-C4 | VGB6 | C57BL/6NTac | |
10301E-H4 | VGB6 | C57BL/6NTac | |