10301   Pramef12     Status: ES cell colonies screened / QC no positives

GenePramef12
Chromosome4
Deletion Start144,395,969
Deletion End144,392,570
Deletion Size3,400
Genome Build38
MGI IDMGI:1924882
NCBI Gene ID77632
BAC ID231A8
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 4

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF144,395,123144,395,143GTTCTGGATTTGCGGGCTTTG
TUP144,395,097144,395,122CACAAGGACTTCTGGACCGTATGGGC
TUR144,395,071144,395,092GAGCGCAAGATGTTGTCGTATC
TDF144,393,012144,393,031GCATCCGACAGCTCAAACAC
TDP144,392,984144,393,010TGAACCTGAGTGGTGTCATTCTGACCG
TDR144,392,957144,392,975GGACTCGAAGAGGCTCAGG

PCR Primers

PrimerSequence
SUACTTCCTGCTTCCTTGGAGC
SDCCACCCATGAAGTAAACACC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev354 bp-++
TUFTUR73 bp++-
TDFTDR75 bp++-
NeoFwdSD369 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
10301E-A6VGB6C57BL/6NTac
10301E-C4VGB6C57BL/6NTac
10301E-H4VGB6C57BL/6NTac