| Gene | Vwa1 |
| Chromosome | 4 |
| Deletion Start | 155,774,499 |
| Deletion End | 155,770,308 |
| Deletion Size | 4,192 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 155,774,420 | 155,774,437 | AGAGCGCGGTAAGTGTGG |
| TUP | 155,774,390 | 155,774,419 | TGAAACCCGGATTCCAATTCTTCATAGCCG |
| TUR | 155,774,346 | 155,774,364 | GGCTGCACCTGGACAGTTC |
| TDF | 155,771,021 | 155,771,044 | CCAGCACTAAACCACCCTTAGATG |
| TDP | 155,770,995 | 155,771,018 | ATGCAGCCACAACAGCTTCATGCC |
| TDR | 155,770,969 | 155,770,988 | CGGAAGCCACTGGACAGAAC |
PCR Primers
| Primer | Sequence |
| SU | ACACCTATATGCCTTTGTGG |
| SD | GTGTGCTCAACTCCACTGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 355 bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 329 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10305A-A10 | VGB6 | C57BL/6NTac | |
| 10305A-H6 | VGB6 | C57BL/6NTac | |
| 10305A-H11 | VGB6 | C57BL/6NTac | |
| 10305A-C7 | VGB6 | C57BL/6NTac | |
