10306 Gpha2 Status: ES cell colonies screened / QC no positives
| Gene | Gpha2 |
| Chromosome | 19 |
| Deletion Start | 6,226,772 |
| Deletion End | 6,227,602 |
| Deletion Size | 831 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 6,226,798 | 6,226,819 | CACCACGAGTCTTGCTCCTTTG |
| TUP | 6,226,821 | 6,226,841 | CTGCTGGGCCTGGCAGTCACT |
| TUR | 6,226,878 | 6,226,900 | CAGAGTTACTCACGGTGCAAGTG |
| TDF | 6,227,463 | 6,227,484 | GGGTGGAATGGGAACAAACTTG |
| TDP | 6,227,487 | 6,227,509 | TCCTCCTCCAACAGGTGAGGGTG |
| TDR | 6,227,574 | 6,227,595 | CGGGAGAAACGGCACATATCAC |
PCR Primers
| Primer | Sequence |
| SU | TGCATAGGATGACATATAAG |
| SD | GGGAGGTCACAGTAATCATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 328 bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 133 bp | + | + | - |
| NeoFwd | SD | 316 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
